Bioss Inc.'s DYKDDDDK (10A2) Monoclonal Antibody is a Rabbit monoclonal antibody. It reacts with Human, Mouse, Rat, Amoeba/Protozoa, Bacteria/Archaea, Bovine, C. elegans/Worm, Canine, Chicken/Bird, Donkey, Drosophila/Arthropod, Feline, Goat, Guinea Pig, Hamster, Horse, Mollusc, Non-Human Primate, Plant, Porcine, Reptile, Rabbit, Sheep, Virus, Xenopus/Amphibian, Yeast/Fungi, Zebrafish/Fish, Other Invertebrate, and Other Mammalian. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blot.
Description
DYKDDDDK polypeptide tag (i.e. FLAG tag, currently owned by Sigma) is a common protein tag added to recombinant proteins. DYKDDDDK was originally used by Munro and Pelham in 1984. This artificially designed tag facilitates the identification and purification of recombinant proteins. The hydrophilic nature of DYKDDDDK make it less likely to denature or inactivate proteins to which it is fused. Moreover, N-terminal FLAG tags can be readily cleaved from proteins with enterokinase. It is one of the most widely used protein tags